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human primary epidermal melanocyte cell line hemn  (ATCC)


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    ATCC human primary epidermal melanocyte cell line hemn
    Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal <t>melanocyte</t> cell line <t>(HEMn),</t> and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.
    Human Primary Epidermal Melanocyte Cell Line Hemn, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+epidermal+melanocyte+cell+line+hemn/pmc07600613-241-17-35?v=ATCC
    Average 94 stars, based on 68 article reviews
    human primary epidermal melanocyte cell line hemn - by Bioz Stars, 2026-08
    94/100 stars

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    1) Product Images from "The In Vitro and In Vivo Anticancer Properties of Chalcone Flavokawain B through Induction of ROS-Mediated Apoptotic and Autophagic Cell Death in Human Melanoma Cells"

    Article Title: The In Vitro and In Vivo Anticancer Properties of Chalcone Flavokawain B through Induction of ROS-Mediated Apoptotic and Autophagic Cell Death in Human Melanoma Cells

    Journal: Cancers

    doi: 10.3390/cancers12102936

    Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.
    Figure Legend Snippet: Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.

    Techniques Used: Derivative Assay, MTT Assay, Microscopy, Expressing, Western Blot, Control, Standard Deviation



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    human primary epidermal melanocyte cell line hemn  (ATCC)
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    ATCC human primary epidermal melanocyte cell line hemn
    Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal <t>melanocyte</t> cell line <t>(HEMn),</t> and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.
    Human Primary Epidermal Melanocyte Cell Line Hemn, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+epidermal+melanocyte+cell+line+hemn/pmc07600613-241-17-35?v=ATCC
    Average 94 stars, based on 1 article reviews
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    human normal epidermal melanocytes cell line hemn lp  (ATCC)
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    ATCC human normal epidermal melanocytes cell line hemn lp
    Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal <t>melanocyte</t> cell line <t>(HEMn),</t> and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.
    Human Normal Epidermal Melanocytes Cell Line Hemn Lp, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+epidermal+melanocyte+cell+line+hemn/pm28328082-34-9-19?v=ATCC
    Average 94 stars, based on 1 article reviews
    human normal epidermal melanocytes cell line hemn lp - by Bioz Stars, 2026-08
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    Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.

    Journal: Cancers

    Article Title: The In Vitro and In Vivo Anticancer Properties of Chalcone Flavokawain B through Induction of ROS-Mediated Apoptotic and Autophagic Cell Death in Human Melanoma Cells

    doi: 10.3390/cancers12102936

    Figure Lengend Snippet: Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.

    Article Snippet: Human epithelial melanoma cell line (A375), human skin lymph node derived melanoma cell line (A2058 [ ]), human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Derivative Assay, MTT Assay, Microscopy, Expressing, Western Blot, Control, Standard Deviation